The Reds made history in Bedfordshire this afternoon with a 2-1 victory; their sixth successive away win!
A Daryl Dike brace saw the Reds back to winning ways at Kenilworth Road.
Goals in either half from the USMNT international were enough for Valérien Ismaël's side who remain fifth in the Sky Bet Championship table.
Whilst kicking off on the front foot, constantly pressing and dominating the ball, the Reds weren't able to create a clear-cut opening in the first 10 minutes.
It was the hosts who had the first effort of note, with around 14 minutes played as Kiernan Dewsbury-Hall hit a sweet volley from 20 yards out that was well held, low down by Brad Collins.
Cauley Woodrow tried his luck soon after, from just outside the box and to the left but his bending effort was a yard away from the top corner.
The Reds were beginning to show their quality midway through the half but some of the technical elements of their play were letting them down and denying them when in good attacking positions.
But Callum Brittain forced Simon Sluga into a save with an effort from 12 yards out before the breakthrough arrived on 27 minutes.
Great interplay from Victor Adeboyejo and Woodrow in the final third saw the latter release the on-running Daryl Dike and the American international powered forward and finished with aplomb from just inside the box to give the Reds a deserved lead heading into the break.
HALF-TIME: Luton 0-1 Barnsley
The early stages of the second period were rather stale but things became livelier with the introduction of two Reds subs, as Aapo Halme (Romal Palmer) and Carlton Morris (Adeboyejo) took to the pitch.
Just a couple of minutes later the lead was doubled as a lovely jinking Woodrow run towards the box ended with his shot being only parried by Sluga and there was Dike to grab his second of the game from close range.
Callum Styles then drove an effort into the far corner that Sluga turned around the posts in spectacular fashion as the Reds continued to dominate in the April sunshine.
After a sterling shift up top and involvement in both goals, Woodrow made way for Austrian forward Dominik Frieser as the Reds looked to see out the win.
The Reds should have gone 3-0 up when Dike was felled in the box, but ended up conceding.
The striker opted to take it himself looking for a hat-trick but Sluga saved low down and the Hatters went straight up the other end and substitute James Collins rattled home for the hosts to make it 2-1.
Luton piled forward in the closing stages trying everything for an equaliser but Ismaël's men saw out the onslaught and recorded their 10th away win of the season, to move onto 68 points and maintain their charge for a playoff place in May.
FULL-TIME: Luton 1-2 Barnsley
Collins, Brittain, Sibbick, Helik, Andersen, Styles, Mowatt, Palmer (Halme), Woodrow (Frieser), Dike, Adeboyejo (Morris).
Unused: Walton, Oduor, J.Williams, Sollbauer, Chaplin.