"It's always an extra bit of a hit when it comes so late and the manner it came," said Wilson of Vale's 89th-minute winner.
"The fact young Mark Marshall danced around us like he did and we didn't put a glove on him - it hurts a little bit more because it was avoidable.
"It typified a lot of the play and our defensive work today as we weren't tight enough. We weren't tight enough all over the pitch and that's a concern for me."
Conor Hourihane equalised from the spot for the Reds after Michael O'Connor's free-kick had sent the hosts into a half-time lead.
"The one thing I'm sure the lads will take from it is that this type of game typifies League One games," added Wilson. "You've got to be committed, you've got to be energetic, you've got to have enthusiasm and be prepared to put your head in where it hurts. With all those things you've got to make sure you earn that right to play the way we want to play. It won't be given to us.
"From our perspective, we have to make sure when we approach the game next week against Swindon will include all those things.
"You've got to go get your tackles in and put your headers in. That's the essence of League One and after that you can go and play."
