The Barnsley boss may have a clean bill of health as far as his squad is concerned, but Ryan Williams, Marcello Trotta and Paul Digby are all away on international duty.
"We're hoping that they're not going to be a miss," said Wilson. "They've contributed a lot, I have to say - particularly Ryan who has played most games for us. We're hoping we have enough in the squad to cope with it.
"There will be a little tinkering here and there but let's hope we don't miss them too much.
"We have got options and that's always been important. You can cahnge the dynamic of the team with what's on your bench. Too many times before when you look at the bench and you can't really change the direction that the game's going in. Now I feel we can. We've got different aspects of the pitch in which we can affect."
Barnsley are aiming for their third consecutive clean sheet as Colchester visit tomorrow night, with the Reds flying after a 5-0 win over Burton Albion in the FA Cup last week.
"The confidence is improved over recent results as you would expect," continued Wilson. "We've played very well at home in recent times, let's hope we can continue that. We never take anything for granted.
"Across the back-line we've done very well, including the goalkeeper as well. We've looked solid and as strong as we have been at any part of the season so far. Long may that continue because, like I've said before, you've got to get your foundations right if you're going to get results and I think in recent weeks we've done that."
The U's visit Oakwell currently in 18th position - two points behind the Reds, and the Barnsley boss isn't taking anything for granted.
"The expectation level of us beating Colchester is there from a lot of people, but we know what type of opposition we're up against and it's going to be a very testing game for us," said Wilson.
"They've had some very good results in recent weeks.
"I’ve seen them over the last couple of seasons and they’ve done very, very well. I know they lost Joe Dunne as a Manager the other month, who did very well with them. They’ve kicked on again with Tony Humes and they look to be a very compact and offensive unit at times."
