The tie will be played Tuesday 6th August 2013, kick-off 7.45pm.
The two sides have only met in the League Cup twice before in a two legged first round fixture in 1980. The Reds won the home tie 2-1 with Banks and Glavin on the scoresheet in front of a 8,430 crowd. Barnsley also won the other leg 1-0 at Scunthorpe.
Scunthorpe United were relegated last season to League Two falling just three points off the safety zone.
First round draw in full:
Bury v Crewe Alexandra
Shrewsbury Town v Bolton Wanderers
Middlesbrough v Accrington Stanley
Doncaster Rovers v Rochdale
Preston North End v Blackpool
Barnsley v Scunthorpe United
Tranmere Rovers v Mansfield Town
York City v Burnley
Notts County v Fleetwood Town
Sheffield United v Burton Albion
Oldham Athletic v Derby County
Carlisle United v Blackburn Rovers
Morecambe v Wolverhampton Wanderers
Nottingham Forest v Hartlepool United
Leeds United v Chesterfield
Huddersfield Town v Bradford City
Rotherham United v Sheffield Wednesday
Port Vale v WalsallGillingham v Bristol City
AFC Bournemouth v Portsmouth
Wycombe Wanderers v Leicester City
Brentford v Dagenham and Redbridge
Exeter City v Queens Park Rangers
Charlton Athletic v Oxford United
Southend United v Yeovil Town
Millwall v AFC Wimbledon
Swindon Town v Torquay United
Birmingham City v Plymouth Argyle
Stevenage v Ipswich Town
Cheltenham Town v Crawley Town
Bristol Rovers v Watford
Northampton Town v Milton Keynes Dons
Leyton Orient v Coventry City
Colchester United v Peterborough United
Brighton and Hove Albion v Newport County