The first round draw is seeded based on the final league positions of teams from the 2012/13 season, and then split regionally - north and south.
As a seeded team, Barnsley will play one of the sides in the non-seeded North list.
Seeds - North
1. Barnsley
2. Blackburn Rovers
3. Blackpool
4. Bolton Wanderers
5. Burnley
6. Crewe Alexandra
7. Derby County
8. Doncaster Rovers
9. Huddersfield Town
10. Leeds United
11. Middlesbrough
12. Nottingham Forest
13. Notts County
14. Sheffield United
15. Sheffield Wednesday
16. Tranmere Rovers
17. Walsall
18. Wolverhampton Wanderers
Non-seeds - North
19. Accrington Stanley
20. Bradford City
21. Burton Albion
22. Bury
23. Carlisle United
24. Chesterfield
25. Fleetwood Town
26. Hartlepool United
27. Mansfield Town
28. Morecambe
29. Oldham Athletic
30. Port Vale
31. Preston North End
32. Rochdale
33. Rotherham United
34. Scunthorpe United
35. Shrewsbury Town
36. York City